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easyseptm mouse treg positive selection kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc easyseptm mouse treg positive selection kit
    Flow cytometric analysis of the purity of sorted CD4 + CD25 high+ thymocytes and splenocytes after negative selection of CD4 + cells followed by CD25 + positive selection of <t>Treg</t> cells. Foxp3 intracellular staining was used to test the purity of Treg subpopulations. The figure shows representative flow cytometric plots
    Easyseptm Mouse Treg Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easyseptm+mouse+treg+positive+selection+kit/pmc07527366-60-23-29?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    easyseptm mouse treg positive selection kit - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells"

    Article Title: Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells

    Journal: Apoptosis

    doi: 10.1007/s10495-020-01629-x

    Flow cytometric analysis of the purity of sorted CD4 + CD25 high+ thymocytes and splenocytes after negative selection of CD4 + cells followed by CD25 + positive selection of Treg cells. Foxp3 intracellular staining was used to test the purity of Treg subpopulations. The figure shows representative flow cytometric plots
    Figure Legend Snippet: Flow cytometric analysis of the purity of sorted CD4 + CD25 high+ thymocytes and splenocytes after negative selection of CD4 + cells followed by CD25 + positive selection of Treg cells. Foxp3 intracellular staining was used to test the purity of Treg subpopulations. The figure shows representative flow cytometric plots

    Techniques Used: Selection, Staining

    a Treg cells express higher level of apoptosis-related proteins than CD4 + T cells. Analysis of 21 apoptosis-related protein expression in the cell lysates of untreated, sorted pTreg cells and CD4 + splenocytes. Bars show the average OD values of each analyte from at least three independent experiments (n = 6). b Effect of DX treatment on the expression of 21 apoptosis-related molecules in T cell subpopulations. Protein arrays were used to detect the in vitro DX treatment (4 h) induced changes of apoptosis-related molecules in cell lysates of sorted CD4 + T cells and pTreg cells. Bars show the DX-induced relative changes (%) of the average OD values of each analyte compared to untreated controls from at least three independent experiments (n = 6). Relative changes were calculated with the following formula: [(OD DX – OD Ctrl )/OD Ctrl ] × 100%
    Figure Legend Snippet: a Treg cells express higher level of apoptosis-related proteins than CD4 + T cells. Analysis of 21 apoptosis-related protein expression in the cell lysates of untreated, sorted pTreg cells and CD4 + splenocytes. Bars show the average OD values of each analyte from at least three independent experiments (n = 6). b Effect of DX treatment on the expression of 21 apoptosis-related molecules in T cell subpopulations. Protein arrays were used to detect the in vitro DX treatment (4 h) induced changes of apoptosis-related molecules in cell lysates of sorted CD4 + T cells and pTreg cells. Bars show the DX-induced relative changes (%) of the average OD values of each analyte compared to untreated controls from at least three independent experiments (n = 6). Relative changes were calculated with the following formula: [(OD DX – OD Ctrl )/OD Ctrl ] × 100%

    Techniques Used: Expressing, In Vitro

    Effect of DX on basal Ca 2+ levels in tTreg and pTreg cells. Short-term (30 min) DX treatment induced elevation of basal cytosolic free Ca 2+ levels in thymic Treg cells. In both organs, the baseline Ca 2+ levels were higher in Treg cells than in the corresponding CD4 + T cells. The columns show the Fluo 3-AM fluorescence intensities (MFI) of Tregs minus MFI of CD4 + T cells. Data represent mean ± SEM (n = 4). Significant change is indicated with asterisk ( p < 0.05)
    Figure Legend Snippet: Effect of DX on basal Ca 2+ levels in tTreg and pTreg cells. Short-term (30 min) DX treatment induced elevation of basal cytosolic free Ca 2+ levels in thymic Treg cells. In both organs, the baseline Ca 2+ levels were higher in Treg cells than in the corresponding CD4 + T cells. The columns show the Fluo 3-AM fluorescence intensities (MFI) of Tregs minus MFI of CD4 + T cells. Data represent mean ± SEM (n = 4). Significant change is indicated with asterisk ( p < 0.05)

    Techniques Used: Fluorescence

    Calcium response is low in thymic and splenic Treg cells. Effect of TCR (anti-CD3) stimulation in thymic CD4 SP and tTreg cells was measured in the presence or absence of DX ( a ). Calcium influx kinetics in splenic CD4 + and Treg cells upon specific TCR-mediated activation (anti-CD3) in the presence or absence of DX ( b ). Data were calculated as the relative ratio of calcium values of Fluo 3-AM fluorescence intensities (median). Columns show the relative ratio of peak to baseline calcium flux measured as Fluo 3-AM fluorescence intensities (MFI) ( c ). The graphs show the mean ± SEM of at least three independent experiments (n = 9). Significant changes are indicated with asterisk (* p < 0.05, ***p < 0.001)
    Figure Legend Snippet: Calcium response is low in thymic and splenic Treg cells. Effect of TCR (anti-CD3) stimulation in thymic CD4 SP and tTreg cells was measured in the presence or absence of DX ( a ). Calcium influx kinetics in splenic CD4 + and Treg cells upon specific TCR-mediated activation (anti-CD3) in the presence or absence of DX ( b ). Data were calculated as the relative ratio of calcium values of Fluo 3-AM fluorescence intensities (median). Columns show the relative ratio of peak to baseline calcium flux measured as Fluo 3-AM fluorescence intensities (MFI) ( c ). The graphs show the mean ± SEM of at least three independent experiments (n = 9). Significant changes are indicated with asterisk (* p < 0.05, ***p < 0.001)

    Techniques Used: Activation Assay, Fluorescence



    Similar Products

    easyseptm mouse treg positive selection kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc easyseptm mouse treg positive selection kit
    Flow cytometric analysis of the purity of sorted CD4 + CD25 high+ thymocytes and splenocytes after negative selection of CD4 + cells followed by CD25 + positive selection of <t>Treg</t> cells. Foxp3 intracellular staining was used to test the purity of Treg subpopulations. The figure shows representative flow cytometric plots
    Easyseptm Mouse Treg Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easyseptm+mouse+treg+positive+selection+kit/pmc07527366-60-23-29?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    easyseptm mouse treg positive selection kit - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Flow cytometric analysis of the purity of sorted CD4 + CD25 high+ thymocytes and splenocytes after negative selection of CD4 + cells followed by CD25 + positive selection of Treg cells. Foxp3 intracellular staining was used to test the purity of Treg subpopulations. The figure shows representative flow cytometric plots

    Journal: Apoptosis

    Article Title: Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells

    doi: 10.1007/s10495-020-01629-x

    Figure Lengend Snippet: Flow cytometric analysis of the purity of sorted CD4 + CD25 high+ thymocytes and splenocytes after negative selection of CD4 + cells followed by CD25 + positive selection of Treg cells. Foxp3 intracellular staining was used to test the purity of Treg subpopulations. The figure shows representative flow cytometric plots

    Article Snippet: In some experiments the CD4 + T cells were subjected to further positive selection to obtain CD4 + CD25 + Treg cells using EasySepTM Mouse Treg Positive Selection Kit (Stemcell Technologies, Vancouver, Canada) following the manufacturer’s instructions.

    Techniques: Selection, Staining

    a Treg cells express higher level of apoptosis-related proteins than CD4 + T cells. Analysis of 21 apoptosis-related protein expression in the cell lysates of untreated, sorted pTreg cells and CD4 + splenocytes. Bars show the average OD values of each analyte from at least three independent experiments (n = 6). b Effect of DX treatment on the expression of 21 apoptosis-related molecules in T cell subpopulations. Protein arrays were used to detect the in vitro DX treatment (4 h) induced changes of apoptosis-related molecules in cell lysates of sorted CD4 + T cells and pTreg cells. Bars show the DX-induced relative changes (%) of the average OD values of each analyte compared to untreated controls from at least three independent experiments (n = 6). Relative changes were calculated with the following formula: [(OD DX – OD Ctrl )/OD Ctrl ] × 100%

    Journal: Apoptosis

    Article Title: Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells

    doi: 10.1007/s10495-020-01629-x

    Figure Lengend Snippet: a Treg cells express higher level of apoptosis-related proteins than CD4 + T cells. Analysis of 21 apoptosis-related protein expression in the cell lysates of untreated, sorted pTreg cells and CD4 + splenocytes. Bars show the average OD values of each analyte from at least three independent experiments (n = 6). b Effect of DX treatment on the expression of 21 apoptosis-related molecules in T cell subpopulations. Protein arrays were used to detect the in vitro DX treatment (4 h) induced changes of apoptosis-related molecules in cell lysates of sorted CD4 + T cells and pTreg cells. Bars show the DX-induced relative changes (%) of the average OD values of each analyte compared to untreated controls from at least three independent experiments (n = 6). Relative changes were calculated with the following formula: [(OD DX – OD Ctrl )/OD Ctrl ] × 100%

    Article Snippet: In some experiments the CD4 + T cells were subjected to further positive selection to obtain CD4 + CD25 + Treg cells using EasySepTM Mouse Treg Positive Selection Kit (Stemcell Technologies, Vancouver, Canada) following the manufacturer’s instructions.

    Techniques: Expressing, In Vitro

    Effect of DX on basal Ca 2+ levels in tTreg and pTreg cells. Short-term (30 min) DX treatment induced elevation of basal cytosolic free Ca 2+ levels in thymic Treg cells. In both organs, the baseline Ca 2+ levels were higher in Treg cells than in the corresponding CD4 + T cells. The columns show the Fluo 3-AM fluorescence intensities (MFI) of Tregs minus MFI of CD4 + T cells. Data represent mean ± SEM (n = 4). Significant change is indicated with asterisk ( p < 0.05)

    Journal: Apoptosis

    Article Title: Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells

    doi: 10.1007/s10495-020-01629-x

    Figure Lengend Snippet: Effect of DX on basal Ca 2+ levels in tTreg and pTreg cells. Short-term (30 min) DX treatment induced elevation of basal cytosolic free Ca 2+ levels in thymic Treg cells. In both organs, the baseline Ca 2+ levels were higher in Treg cells than in the corresponding CD4 + T cells. The columns show the Fluo 3-AM fluorescence intensities (MFI) of Tregs minus MFI of CD4 + T cells. Data represent mean ± SEM (n = 4). Significant change is indicated with asterisk ( p < 0.05)

    Article Snippet: In some experiments the CD4 + T cells were subjected to further positive selection to obtain CD4 + CD25 + Treg cells using EasySepTM Mouse Treg Positive Selection Kit (Stemcell Technologies, Vancouver, Canada) following the manufacturer’s instructions.

    Techniques: Fluorescence

    Calcium response is low in thymic and splenic Treg cells. Effect of TCR (anti-CD3) stimulation in thymic CD4 SP and tTreg cells was measured in the presence or absence of DX ( a ). Calcium influx kinetics in splenic CD4 + and Treg cells upon specific TCR-mediated activation (anti-CD3) in the presence or absence of DX ( b ). Data were calculated as the relative ratio of calcium values of Fluo 3-AM fluorescence intensities (median). Columns show the relative ratio of peak to baseline calcium flux measured as Fluo 3-AM fluorescence intensities (MFI) ( c ). The graphs show the mean ± SEM of at least three independent experiments (n = 9). Significant changes are indicated with asterisk (* p < 0.05, ***p < 0.001)

    Journal: Apoptosis

    Article Title: Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4 + T cells

    doi: 10.1007/s10495-020-01629-x

    Figure Lengend Snippet: Calcium response is low in thymic and splenic Treg cells. Effect of TCR (anti-CD3) stimulation in thymic CD4 SP and tTreg cells was measured in the presence or absence of DX ( a ). Calcium influx kinetics in splenic CD4 + and Treg cells upon specific TCR-mediated activation (anti-CD3) in the presence or absence of DX ( b ). Data were calculated as the relative ratio of calcium values of Fluo 3-AM fluorescence intensities (median). Columns show the relative ratio of peak to baseline calcium flux measured as Fluo 3-AM fluorescence intensities (MFI) ( c ). The graphs show the mean ± SEM of at least three independent experiments (n = 9). Significant changes are indicated with asterisk (* p < 0.05, ***p < 0.001)

    Article Snippet: In some experiments the CD4 + T cells were subjected to further positive selection to obtain CD4 + CD25 + Treg cells using EasySepTM Mouse Treg Positive Selection Kit (Stemcell Technologies, Vancouver, Canada) following the manufacturer’s instructions.

    Techniques: Activation Assay, Fluorescence